This product is n - (5-chloro-3-pyrazinyl) - 4-aminobenzenesulfonamide sodium salt monohydrate,
which contains at least 99.0% c10h8cln4nao2s · H2O.
Character:
This product is a white or light yellow powder, tasteless.It is soluble in water or methanol, slightly
soluble in ethanol or acetone, and insoluble in chloroform.
Identification:
(1) take this product, add methanol to make a solution containing 1mg of sodium
Sulfachloropyrazine per 1ml, as the test solution; take sodium Sulfachloropyrazine as the control
solution, add methanol to make a solution containing 1mg of sodium Sulfachloropyrazine per 1ml,
as the control solution, absorb 2 μ l of each of the above two solutions, and test the color of the
main spots of the test solution according to the method under related substances It should be the
same as the main spot of the reference solution.
(2) The infrared absorption spectrum of this product should be consistent with the control spectrum
(SOP - 8043).
Inspection:
The particle size of the product should not exceed 1% when it is larger than 500 μ m, and should not
be less than 17% when it is 500-40 μ m (sop-8027).
PH value:
Take this product, add water to make 5% solution, and determine according to the law (SOP - 8008).
The pH value should be 9.0-10.5.
The clarity of the solution:
2.5G, add 50ml of water to dissolve, and the solution should be clarified (sop-8015).
Related substances:
Take this product and add methanol to make a solution containing 10mg of sulfachlorpyridazine
sodium per 1ml for test .The solution containing 0.3mg per 1ml was made by adding methanol to
the sample solution, which was used as the control solution. According to the test of thin layer
chromatography (SOP - 8022), absorb 2 μ l of each of the above two solutions, respectively point
them on the same silica gel G thin layer plate, use toluene - ethyl acetate - methanol (3:1:1) as the
developing agent, and then dry them in the air. Place the thin plate in a closed container filled with
nitrous acid gas (2 g sodium nitrite, 5 ml hydrochloric acid), take it out after 5 minutes, place it at
room temperature for 3 - 5 minutes, spray with 0.1% naphthalene ethylenediamine hydrochloride
solution, if impurity spots appear in the test solution, the color shall not be deeper than the main
spot of the control solution.
Water content:
Take about 0.5g of this product, weigh it precisely, add 20ml of anhydrous methanol, close it, shake
it for 10 minutes, and determine according to the law (SOP - 8012). The moisture content shall not
exceed 6.5%.
Heavy metals:
In 1g of the product were determined according to the law (SOP - 8017, the third method). The
content of heavy metals should not exceed 20 ppm.
Arsenate:
Take 1g of arsenic salt, first carbonize it with low fire, then incinerate it completely at 500-600 ºC,
cool it, add 5ml hydrochloric acid and 23ml water to dissolve it, and check according to the law (SOP
- 8019), which should meet the requirements (0.0005%).
Content determination:
Take about 0.5g of this product, accurately weigh it, add 40ml of water to dissolve it, add 40ml of
dimethylformamide, shake well, slowly add 25ml of hydrobromic acid (1 → 2) solution, and titrate
with sodium nitrite solution (0.1mol/l) according to the perpetual stop titration method (SOP -
8009). Each 1ml of sodium nitrite solution (0.1mol/l) is equivalent to 32.47mg of
c10h8cln4nao2s · H2O.
Function and use:
Sulfonamides. It is mainly used for coccidiosis.
Storage:
keep out of light and sealed.
Retest period:
The first three months of validity.
Validity:
2 years
Standard source:
Chinese Veterinary Medicine Dictionary (2015 Edition)
Packaging:
Audited Supplier 
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